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PubMed · 6155271

Delayed-type hypersensitivity induced by antigen-pulsed, bone marrow-derived macrophages.

P L Mottram·J F Miller
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Abstract

Mouse bone marrow cells, cultured in specifically conditioned medium, were found to be virtually pure macrophages after 7 days. When pulsed with antigen, these cells induced and elicited delayed-type hypersensitivity (DTH) with the same efficiency and major histocompatibility complex restrictions as antigen-pulsed peritoneal exudate cells. Both of these macrophage populations had 20% Ia+ cells, as measured by complement-mediated cytotoxicity. When most of the Ia+ cells were removed, the remaining antigen-pulsed macrophages could not elicit DTH in sensitized mice.

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AminopeptidasesAnimalsAntigensBone Marrow CellsCytotoxicity, ImmunologicFemaleHemocyaninsHypersensitivity, DelayedImmunity, CellularMacrophagesMicePeptidesStaining and LabelingJournal Article

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P L Mottram, J F Miller. 1980. Delayed-type hypersensitivity induced by antigen-pulsed, bone marrow-derived macrophages.. https://doi.org/10.1002/eji.1830100302

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