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Interferon induction by viruses. Sindbis virus: defective-interfering particles temperature-sensitive for interferon induction.

F J Fuller·P I Marcus
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Abstract

A defective-interfering (DI) particle of Sindbis virus was generated from a ts mutant of RNA-complementation group A by serially undiluted passages at 30 degrees C. This mutant induced interferon at a permissive temperature (30 degrees C), but not at a non-permissive temperature (40.5 degrees C); it also expressed homotypic interference throughout the range 30 to 40.5 degrees C. This demonstrates for the first time in a DI particle a ts function, namely, the ability to induce interferon. In addition, our data provide further evidence that the RNA genome of a Sindbis DI particle can be translated within the cell. We postulate that the products of translation function to produce the putative inducer of interferon, namely a molecule of dsRNA.

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Defective VirusesGenes, ViralInterferonsMutationProtein BiosynthesisRNA, ViralSindbis VirusTemperatureViral InterferenceJournal ArticleResearch Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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F J Fuller, P I Marcus. 1980. Interferon induction by viruses. Sindbis virus: defective-interfering particles temperature-sensitive for interferon induction.. https://doi.org/10.1099/0022-1317-48-2-391

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