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PubMed · 6158095

A technique for expressing eukaryotic genes in bacteria.

L Guarente·T M Roberts·M Ptashne
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Abstract

Methods are described that allow efficient expression in Escherichia coli of cloned eukaryotic genes. The methods require that the coding sequence of the gene in question be available in a form uninterrupted by intervening sequences (for example, as a complementary DNA clone). The gene products are synthesized unfused to other amino acid sequences. The genetic manipulations are simple, and require the plasmids described and commercially available enzymes.

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Cloning, MolecularDNA, BacterialEscherichia coliGenesGlobinsInterferonsOperonPeptide Chain Initiation, TranslationalPlasmidsProtein BiosynthesisTranscription, GeneticViral ProteinsJournal Article

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L Guarente, T M Roberts, M Ptashne. 1980-09-19. A technique for expressing eukaryotic genes in bacteria.. https://doi.org/10.1126/science.6158095

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