Human leukocyte interferon produced by E. coli is biologically active.

D V Goeddel; E Yelverton; A Ullrich; H L Heyneker; G Miozzari; W Holmes; P H Seeburg; T Dull; L May; N Stebbing; R Crea; S Maeda; R McCandliss; A Sloma; J M Tabor; M Gross; P C Familletti; S Pestka

doi:10.1038/287411a0

PubMed · 6159538

Human leukocyte interferon produced by E. coli is biologically active.

D V Goeddel·E Yelverton·A Ullrich·H L Heyneker·G Miozzari·W Holmes·P H Seeburg·T Dull·L May·N Stebbing·R Crea·S Maeda·R McCandliss·A Sloma·J M Tabor·M Gross·P C Familletti·S Pestka

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Abstract

A human leukocyte interferon cDNA was enzymatically synthesized, inserted into the vector pBR322, and cloned in Escherichia coli. The DNA sequence codes for a 23-amino acid signal peptide followed by an interferon polypeptide of 165 amino acids. An expression plasmid was constructed which permits the synthesis in E. coli of 2.5 x 10(8) units of interferon per litre of culture. This LeIF protected squirrel monkeys from lethal encephalomyocarditis virus infection.

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Amino Acid Sequence Cloning, Molecular DNA, Recombinant Escherichia coli Humans Interferons Leukocytes Plasmids Protein Precursors Journal Article

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D V Goeddel, E Yelverton, A Ullrich, H L Heyneker, G Miozzari, W Holmes, P H Seeburg, T Dull, L May, N Stebbing, R Crea, S Maeda, R McCandliss, A Sloma, J M Tabor, M Gross, P C Familletti, S Pestka. 1980-10-02. Human leukocyte interferon produced by E. coli is biologically active.. https://doi.org/10.1038/287411a0

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