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PubMed · 6163067

[Model experiments for quantitative cytophotometry using protein films (author's transl)].

G Desoye·E Schauenstein
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Abstract

The usefulness of protein films denaturated and fixed with ethanol/ether as cytochemical model systems was investigated. Kinetics of staining with amido black 10B were analyzed as well as the spectroscopic properties of the protein-dye complex. The absorption coefficient of the complex was estimated. Data obtained so far are compared with both stained cells and the protein-dye complex in solution. Discussion focuses on the influence of fixation and the possibilities to use the films as systems for calibrating cytochemical stainings.

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AnimalsCarcinoma, Ehrlich TumorHistocytochemistryProteinsReference StandardsSpectrophotometryStaining and LabelingEnglish AbstractJournal Article

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G Desoye, E Schauenstein. 1981. [Model experiments for quantitative cytophotometry using protein films (author's transl)].. https://pubmed.ncbi.nlm.nih.gov/6163067/

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