Interaction between Serratia protease and human plasma alpha 2 macroglobulin.

K Miyata; M Nakamura; K Tomoda

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PubMed · 6166607

Interaction between Serratia protease and human plasma alpha 2 macroglobulin.

K Miyata·M Nakamura·K Tomoda

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Abstract

Serratia protease (TSP) [EC 3.4.24] was bound stoichiometrically to alpha 2 macroglobulin (alpha 2M), which was purified and crystallized from human plasma, but apo TSP was not bound. On formation of the TSP-alpha 2M complex the enzymatic activity of the bound TSP was affected with respect to substrates; Km values of the bound TSP were unchanged but Vmax values were reduced. alpha 2M was cleft at the mid-region of its subunits chains by TSP, which resulted in a conformational change of the alpha 2M molecule with TSP.

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Electrophoresis, Polyacrylamide Gel Endopeptidases Humans Kinetics Metalloendopeptidases Protein Conformation Serratia Spectrophotometry, Ultraviolet alpha-Macroglobulins Journal Article

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K Miyata, M Nakamura, K Tomoda. 1981. Interaction between Serratia protease and human plasma alpha 2 macroglobulin.. https://pubmed.ncbi.nlm.nih.gov/6166607/

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