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Large globin RNA molecules and their processing.

Y Shaul·J Kaminchik·H Aviv
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Abstract

RNA containing beta-globin message sequences larger than 2000 nucleotides could be detected in nuclei of murine erythroid cells using cloned beta-globin cDNA. Under steady-state conditions, when nuclear RNA was separated on denaturing agarose gels and covalently bound to diazobenzyloxymethyl-paper, a 4200-nucleotide and a approximately equal to 3500-nucleotide band could be seen. The presence of these large molecules could also be visualized under the electron microscope after hybridization to a beta-globin genomic DNA fragment. We suggest that these molecules are precursors to mature mRNAs. In addition to these large molecules, a series of molecules smaller than 2000 nucleotides were seen. These are postulated to be processing intermediates in the maturation of beta-globin mRNA.

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AnimalsCell LineCell NucleusClone CellsGlobinsLeukemia, ExperimentalMiceMice, Inbred DBAMicroscopy, ElectronMolecular WeightNucleic Acid ConformationNucleic Acid HybridizationProtein BiosynthesisRNAJournal Article

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Y Shaul, J Kaminchik, H Aviv. 1981-06-01. Large globin RNA molecules and their processing.. https://doi.org/10.1111/j.1432-1033.1981.tb05358.x

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