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PubMed · 6170207

Methodological approaches to antigen identification in soluble immune complexes; A model study.

S Husby·S E Svehag·H Nielsen·N Høiby·P O Schiøtz
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Abstract

The usefulness of different techniques for identification of immune complex-(IC) bound antigen(s) was investigated in two Ab-Ag model systems. The following approaches were used: 1) determination of IC-activity before and after removal of IC by adsorption to antibody-specific solid phase systems. 2) analysis of PEG-insoluble IC by reference line-rocket immunoelectrophoresis and 3) reprecipitation of PEG-insoluble IC in the presence of 125I-labelled antibody to suspected antigens and subsequent analysis of the distribution of the radioactivity. This latter approach, the use of a double PEG precipitation immunoradiometric assay, was found most useful and simple for identification of antigen(s) contained in soluble IC. The technique is most sensitive in demonstrating IC-bound antigen(s) when the IC are formed in slight to moderate antigen excess.

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AdsorptionAntigen-Antibody ComplexAntigens, BacterialEpitopesHumansImmunoelectrophoresisImmunologic TechniquesRadioimmunoassayJournal ArticleResearch Support, Non-U.S. Gov't

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S Husby, S E Svehag, H Nielsen, N Høiby, P O Schiøtz. 1981. Methodological approaches to antigen identification in soluble immune complexes; A model study.. https://doi.org/10.1111/j.1699-0463.1981.tb02679.x

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