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Human beta interferon gene localization and expression in somatic cell hybrids.

P M Pitha·D L Slate·N B Raj·F H Ruddle
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Abstract

The human fibroblast interferon gene beta 1 was mapped to human chromosome 9. Sequence homology with a beta 1 cDNA clone was detected in both genomic DNA and induced mRNA of human/mouse or human/hamster somatic cell hybrids containing human chromosome 9, but not in lines lacking this chromosome or those retaining a complex translocation involving chromosomes 9 and 11. Interferon mRNA that did not share sequence homology with the beta 1 cDNA clone was detected in lines containing human chromosomes 2 and 5 but lacking chromosome 9, suggesting the presence of other unlinked interferon sequences in the human genome.

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AnimalsBase SequenceChromosomes, Human, 6-12 and XCricetinaeDNAFibroblastsGene Expression RegulationHumansHybrid CellsInterferonsNucleic Acid HybridizationProtein BiosynthesisRNA, MessengerTranslocation, GeneticJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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P M Pitha, D L Slate, N B Raj, F H Ruddle. 1982. Human beta interferon gene localization and expression in somatic cell hybrids.. https://doi.org/10.1128/mcb.2.5.564-570.1982

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