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Oligo(U) sequences present in sea urchin maternal RNA decrease following fertilization.

R Duncan·T Humphreys
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Abstract

Oligo(U) tracts were identified and measured in RNA from sea urchin eggs and embryos using a quantitative assay based on the amount of [3H]poly(A) protected from RNase T2 in duplexes with the oligo(U). The oligo(U) amounted to 0.0035% of egg RNA (0.063 X 10(-12) g/egg) and decreased to 0.0015% (0.027 X 10(-12) g/embryo) by 2 hr after fertilization. The oligo(U) tracts had a maximum size of 15-30 nucleotides and were associated with two size classes of RNA. In eggs about half were in 100 to 200 nucleotide RNA and half in mRNA-sized molecules. After fertilization, the oligo(U) in the population of large-mRNA-sized molecules was greatly reduced.

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AnimalsBase SequenceEmbryo, NonmammalianFemaleFertilizationOligonucleotidesOligoribonucleotidesOvumPoly ARNASea UrchinsUracil NucleotidesJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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R Duncan, T Humphreys. 1983. Oligo(U) sequences present in sea urchin maternal RNA decrease following fertilization.. https://doi.org/10.1016/0012-1606(83)90326-3

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