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Solid-phase radioimmunoassay for substance P.

A Oblin·B Zivkovic
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Abstract

The solid-phase immunoassay for quantification of substance P has been developed. The assay is based on the repartition of anti-substance P antibodies between the insoluble phase-immobilized substance P and the free peptide. The immobilized substance P-antibody complex is then quantified with 125I-protein A. The method allowed detection of 10 pg of substance P. The values of substance P concentration obtained by the present method in different regions of the rat brain were comparable to those obtained by standard radioimmunoassay with 125I-tyr-8-substance P as tracer. The described solid-phase radioimmunoassay is a simple, sensitive, and reliable technique for quantification of substance P-like immunoreactivity in biological samples.

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AnimalsAntigen-Antibody ComplexBrain ChemistryCross ReactionsKineticsRabbitsRadioimmunoassaySubstance PJournal Article

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BibTeXRIS

A Oblin, B Zivkovic. 1983. Solid-phase radioimmunoassay for substance P.. https://doi.org/10.1111/j.1471-4159.1983.tb08047.x

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