Spot blot: a hybridization assay for specific DNA sequences in multiple samples.

M Cunningham

doi:10.1016/0003-2697(83)90395-0

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Spot blot: a hybridization assay for specific DNA sequences in multiple samples.

M Cunningham

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Abstract

Small aliquots of DNA-containing samples from gradient or column fractions are spotted onto agarose plates containing ethidium bromide, and the DNA is visualized qualitatively. After it is overlaid with a thin agarose cover, the DNA is denatured and transferred to nitrocellulose filters for hybridization. This method, called the "spot-blot assay," is particularly convenient when dealing with large numbers of samples.

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Base Sequence DNA Escherichia coli Ethidium Methods Nucleic Acid Hybridization Physarum RNA Journal Article Research Support, Non-U.S. Gov't

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M Cunningham. 1983-02-01. Spot blot: a hybridization assay for specific DNA sequences in multiple samples.. https://doi.org/10.1016/0003-2697(83)90395-0

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