Phospholipid bilayers made from monolayers on patch-clamp pipettes.

R Coronado; R Latorre

doi:10.1016/s0006-3495(83)84343-4

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Phospholipid bilayers made from monolayers on patch-clamp pipettes.

R Coronado·R Latorre

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Abstract

Phospholipid bilayers were made from phospholipid monolayers at the air/water interface on patch-clamp pipettes. Lipid bilayers were characterized using the K+ carrier nonactin and the channel formers gramicidin and alamethicin. Bilayers were also formed from monolayers spontaneously assembled in a suspension of native vesicles from cardiac sarcolemma and lobster axonal membranes and an excess of lipids. In these types of bilayers we observed several different channels including one contained in the axonal membrane that shows delayed rectifier behavior. This technique permits the study of reconstituted channels on a time scale and noise comparable to cellular patch-clamp standards.

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Alamethicin Animals Axons Cattle Cell Membrane Gramicidin In Vitro Techniques Lipid Bilayers Models, Biological Myocardium Nephropidae Phospholipids Potassium Sarcolemma Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

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R Coronado, R Latorre. 1983. Phospholipid bilayers made from monolayers on patch-clamp pipettes.. https://doi.org/10.1016/s0006-3495(83)84343-4

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