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Restriction and modification in Bacillus subtilis: sequence specificities of restriction/modification systems BsuM, BsuE, and BsuF.

S Jentsch
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Abstract

The sequence specificities of three Bacillus subtilis restriction/modification systems were established: (i) BsuM (CTCGAG), an isoschizomer to XhoI; (ii) BsuE (CGCG), an isoschizomer to FnuDII; and (iii) BsuF (CCGG), an isoschizomer to MspI, HpaII. The BsuM modification enzyme methylates the 3' cytosine of the recognition sequence. The BsuF modification enzyme methylates the 5' cytosine of the sequence, rendering such sites resistant to MspI degradation and leaving the majority of sites sensitive to HpaII degradation.

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Bacillus subtilisBacterial ProteinsBase SequenceDNA Restriction EnzymesDNA, BacterialDeoxyribonuclease HpaIIDeoxyribonucleases, Type II Site-SpecificGenesGenes, BacterialMethylationPlasmidsJournal Article

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S Jentsch. 1983. Restriction and modification in Bacillus subtilis: sequence specificities of restriction/modification systems BsuM, BsuE, and BsuF.. https://doi.org/10.1128/jb.156.2.800-808.1983

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