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PubMed · 6195555

Handling Golgi-impregnated tissue for light microscopy.

P J Berbel·A Fairén
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Abstract

The use of cyanocrylic glue to fix pieces of Golgi-stained nervous tissue on a paraffin blank is proposed for obtaining thick sections of unembedded tissue with a sliding microtome. This procedure makes correct orientation of the tissue easy during sectioning and makes it possible to obtain tissue sections quickly. The sections are flat-mounted using epoxy resin, resulting in permanent preparations with excellent optical properties and enabling further thin-sectioning for light and electron microscopic studies.

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AdhesivesAnimalsCerebral CortexEpoxy ResinsInterneuronsMiceMicroscopyMicroscopy, ElectronMicrotomyParaffinStaining and LabelingJournal ArticleResearch Support, Non-U.S. Gov't

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P J Berbel, A Fairén. 1983-08-08. Handling Golgi-impregnated tissue for light microscopy.. https://doi.org/10.1016/0304-3940(83)90369-5

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PubMed2007

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