Identifier sequences are transcribed specifically in brain.

J G Sutcliffe; R J Milner; J M Gottesfeld; R A Lerner

doi:10.1038/308237a0

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PubMed · 6199680

Identifier sequences are transcribed specifically in brain.

J G Sutcliffe·R J Milner·J M Gottesfeld·R A Lerner

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Abstract

'Identifier' or ID sequences are present in 62% of the RNA polymerase II and III transcripts made in vitro from brain nuclei but in fewer than 4% of the transcripts made from the nuclei of other tissues. An homologous 160-nucleotide cytoplasmic poly(A)+ RNA species, BC1, and a smaller species, BC2, are located in vivo exclusively in neural tissues. Cloned ID sequences are polymerase III templates in vitro. Our data suggest a model in which brain-specific polymerase III transcription of ID sequences located in introns of brain genes activates those genes in a primary manner for polymerase III transcription.

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Animals Base Sequence Brain Gene Expression Regulation Operon RNA RNA Polymerase II RNA Polymerase III Rats Transcription, Genetic Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

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J G Sutcliffe, R J Milner, J M Gottesfeld, R A Lerner. Identifier sequences are transcribed specifically in brain.. https://doi.org/10.1038/308237a0

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