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PubMed · 6199869

A method for staining actin-containing structures in thick plastic sections for medium voltage electron microscopy.

J M Smith
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Abstract

A staining procedure has been developed for imaging actin-containing structures in thick plastic sections in the electron microscope. The stress fibres of a fibroblastic cell line were used as a model system, and were first characterized immunocytochemically. After fixation of cells in formaldehyde, mordanting in a solution of gadolinium chloride allows stress fibres to be stained for light microscopy with haematoxylin. A brief exposure to a solution of ammonium paramolybdate renders haematoxylin-stained structures sufficiently electron-dense to be imaged in 1 micron thick plastic sections in a JEOL 200CX electron microscope, operating at 200 kV, and possibly in conventional instruments operating at 100 kV, particularly if equipped with a lanthanum hexaboride source.

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ActinsAnimalsArvicolinaeCell LineFibroblastsMicroscopy, ElectronStaining and LabelingJournal ArticleResearch Support, Non-U.S. Gov't

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J M Smith. 1984. A method for staining actin-containing structures in thick plastic sections for medium voltage electron microscopy.. https://doi.org/10.1016/0040-8166(84)90017-x

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