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Two monoclonal antibodies recognizing determinants on human embryonal carcinoma cells react specifically with the liver isozyme of human alkaline phosphatase.

P W Andrews·L J Meyer·K L Bednarz·H Harris
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Abstract

From a series of hybridomas that produced monoclonal antibodies reactive with the surface of human embryonal carcinoma cells, two that specifically recognized determinants of the liver/bone/kidney isozyme of alkaline phosphatase were isolated. They did not cross-react with the intestinal or placental isozymes. Phylogenetic studies revealed that both antibodies cross-reacted strongly with liver alkaline phosphatase from higher primates, but exhibited marked differences in their respective cross-reactions with liver alkaline phosphatase from other mammalian species.

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Alkaline PhosphataseAnimalsAntibodies, MonoclonalAntibodies, NeoplasmAntigens, NeoplasmCross ReactionsEmbryonal Carcinoma Stem CellsEpitopesHumansIsoenzymesLiverMiceNeoplastic Stem CellsPrimatesSpecies SpecificityStem CellsTeratomaComparative StudyJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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P W Andrews, L J Meyer, K L Bednarz, H Harris. 1984. Two monoclonal antibodies recognizing determinants on human embryonal carcinoma cells react specifically with the liver isozyme of human alkaline phosphatase.. https://doi.org/10.1089/hyb.1984.3.33

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