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PubMed · 6207192

Efficient method for visualization and isolation of proteins resolved in polyacrylamide gels.

R T Francis·J R Davie·M Sayre·E Rocha·F Ziemer·G Riedel
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Abstract

Polyacrylamide gel electrophoresis is a popular method used to purify proteins for reconstitution experiments, amino acid composition and sequence determinations. In this report we describe methods that will be of general use in the isolation and characterization of proteins and the benefits of substituting boric acid for glycine in the electrophoresis tray buffers. We also describe how proteins resolved in a variety of gel systems (including those containing sodium dodecyl sulfate) may be rapidly visualized with 8-anilino-1-naphthalene sulfonic acid and efficiently transferred to a second gel for two-dimensional gel analysis, or isolated by electroelution for subsequent characterization.

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Anilino NaphthalenesulfonatesAnimalsBlood ProteinsBoric AcidsElectrophoresis, Polyacrylamide GelGlycineHistonesHumansProteinsSodium Dodecyl SulfateStaining and LabelingTroutJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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R T Francis, J R Davie, M Sayre, E Rocha, F Ziemer, G Riedel. 1984-08-17. Efficient method for visualization and isolation of proteins resolved in polyacrylamide gels.. https://doi.org/10.1016/s0021-9673(01)92699-8

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