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PubMed · 6220922

Temperature-dependent conformational changes in isolated oligomycin-sensitive ATPase.

G Curatola·R M Fiorini·G Solaini·A Baracca·G Parenti Castelli·G Lenaz
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Abstract

Isolated oligomycin-sensitive ATPase undergoes a kinetic change at 20-25 degrees C with a higher activation energy and a lower Km for ATP below this temperature range. This observation has been correlated with temperature-dependent structural changes detected by circular dichroism in the UV region in the isolated enzyme. The negative ellipticities in the 208-225 nm region, which are proportional to the alpha-helix content, increase with rise in temperature to a maximum above 25 degrees C.

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Adenosine TriphosphatasesAnimalsCattleCircular DichroismKineticsMitochondria, HeartOligomycinsProtein ConformationTemperatureJournal ArticleResearch Support, Non-U.S. Gov't

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G Curatola, R M Fiorini, G Solaini, A Baracca, G Parenti Castelli, G Lenaz. 1983-05-02. Temperature-dependent conformational changes in isolated oligomycin-sensitive ATPase.. https://doi.org/10.1016/0014-5793(83)80224-5

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