Phosphofructokinase is a calmodulin binding protein.

G W Mayr; L M Heilmeyer

doi:10.1016/0014-5793(83)80415-3

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Phosphofructokinase is a calmodulin binding protein.

G W Mayr·L M Heilmeyer

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Abstract

A trial to purify myosin light chain kinase from crude myosin led to the isolation of a Mr 85 000 calmodulin binding protein different from this enzyme. Because it showed inherent phosphofructokinase activity we investigated its relation to this enzyme. We demonstrated identity to phosphofructokinase by a close to identical amino acid composition, by antigenic identity and a set of completely identical peptide maps. The calmodulin binding property was also shown for a fraction of the enzyme prepared by standard methods. First experiments show that Ca2+--calmodulin is a potent regulator of phosphofructokinase polymerization.

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Amino Acids Animals Calmodulin-Binding Proteins Carrier Proteins Cattle Chromatography, Affinity Crystallization Immunodiffusion Molecular Weight Myosin-Light-Chain Kinase Phosphofructokinase-1 Protein Kinases Journal Article Research Support, Non-U.S. Gov't

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G W Mayr, L M Heilmeyer. 1983-08-08. Phosphofructokinase is a calmodulin binding protein.. https://doi.org/10.1016/0014-5793(83)80415-3

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