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Two-dimensional 1H NMR study of the lambda operator site OL1: a sequential assignment strategy and its application.

M A Weiss·D J Patel·R T Sauer·M Karplus
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Abstract

The solution structure and dynamics of the 17-base-pair synthetic operator site OL1, which is recognized by the cI and Cro repressors of bacteriophage lambda, is studied by two-dimensional NMR methods. A sequential assignment strategy for nucleic acids is proposed and illustrated by the assignments of the base and sugar protons of OL1.

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Bacteriophage lambdaBase CompositionDNA-Binding ProteinsMagnetic Resonance SpectroscopyNucleic Acid ConformationOperonProtein ConformationRepressor ProteinsTranscription FactorsViral ProteinsViral Regulatory and Accessory ProteinsJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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M A Weiss, D J Patel, R T Sauer, M Karplus. 1984. Two-dimensional 1H NMR study of the lambda operator site OL1: a sequential assignment strategy and its application.. https://doi.org/10.1073/pnas.81.1.130

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