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PubMed · 6238843

Plasminogen activator activity in differentiating leukemia cells.

M Kidron·I Nachshon·M Mayer·E Fibach
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Abstract

Plasminogen activator (PA) activity of human promyelocytic leukemia cell line HL-60 was assayed by following the conversion of plasminogen to plasmin and the plasmin-mediated hydrolysis of 14C-labeled globin. When HL-60 cells were induced to differentiate into macrophages by 12-O-tetradecanoyl-phorbol-13-acetate (TPA), cell-associated PA activity and secretion of PA into the conditioned medium increased profoundly. PA activity increased earlier and as a result of lower concentrations of TPA than the ability of the cells to adhere. Exposure to 10(-6)M dexamethasone did not prevent TPA-induced adherence and produced a slight inhibition of cellular PA activity. These findings imply that TPA-induced differentiation of HL-60 cells to macrophage-like cells is associated with induction of PA activity.

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Cell AdhesionCell DifferentiationCell LineDexamethasoneFibrinolysinHumansLeukemia, Myeloid, AcutePlasminogenPlasminogen ActivatorsTetradecanoylphorbol AcetateJournal Article

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M Kidron, I Nachshon, M Mayer, E Fibach. 1984-11-05. Plasminogen activator activity in differentiating leukemia cells.. https://doi.org/10.1016/0014-5793(84)80982-5

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