Complement receptors: specific detection by molecular complexes.

J Cossman; J C Glorioso; R Adler

doi:10.1016/0022-1759(78)90182-5

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Complement receptors: specific detection by molecular complexes.

J Cossman·J C Glorioso·R Adler

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Abstract

Immune complexes of BSA-anti BSA (IgG F(ab')2) will activate human complement by the alternate pathway. The C3 bound to these complexes binds to human peripheral blood mononuclear cells which bear complement receptors. This interaction is visualized by fluoresceinated antigen (BSA-FITC) or fluorescein conjugated antisera directed at the C3 component of complement. The assay appears to be more sensitive than the usual IgMEAC rosette technique and correlates well with the rosette method.

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Animals Antibody Specificity Antigen-Antibody Complex Binding Sites Cattle Complement C3 Complement C4 Complement Fixation Tests Complement System Proteins Humans Immunoglobulin Fab Fragments Serum Albumin, Bovine Journal Article Research Support, U.S. Gov't, P.H.S.

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J Cossman, J C Glorioso, R Adler. 1978. Complement receptors: specific detection by molecular complexes.. https://doi.org/10.1016/0022-1759(78)90182-5

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