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Polycistronic vesicular stomatitis virus RNA transcripts.

R C Herman·M Schubert·J D Keene·R A Lazzarini
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Abstract

A procedure to enrich for the sequences present at the junction between the linked messages in the polycistronic RNAs symthesized in vitro by vesicular stomatitis virus (VSV) is described. Analyses of these sequences show that they contain a precise transcript of both the intercistronic dinucleotide and the pentanucleotide 5'--C-U-G-U-U--3', common to the 5'-end of all VSV cistrons, covalently linked to the 3'-side of the intervening poly(A). The data strongly suggest that the VSV transcriptase polyadenylylates the mRNAs and can then resume direct and precise transcription of the genome-without reinitiation and without skipping nucleotides.

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Base SequenceGenesGenes, ViralNucleic Acid PrecursorsPoly ARNA, MessengerRNA, ViralTranscription, GeneticVesicular stomatitis Indiana virusJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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R C Herman, M Schubert, J D Keene, R A Lazzarini. 1980. Polycistronic vesicular stomatitis virus RNA transcripts.. https://doi.org/10.1073/pnas.77.8.4662

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