A simple isolation method for basal-lateral plasma membranes from rat kidney cortex.

V Scalera; Y K Huang; B Hildmann; H Murer

doi:10.3109/09687688109065422

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A simple isolation method for basal-lateral plasma membranes from rat kidney cortex.

V Scalera·Y K Huang·B Hildmann·H Murer

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Abstract

Basal-lateral membranes were separated in a self-orienting Percoll (modified colloidal silica) gradient from a heavy microsomal membrane fraction by centrifugation at 48,000g for 0.5 h. The (Na+--K+)-ATPase activity as a marker enzyme for the basal-lateral plasma membrane was 20-fold enriched by this procedure. The adenylate-cyclase activity measured in the basal-lateral membrane fraction was stimulated 6-fold by parathyrin and only up to 1.5-fold by arginine-vasopressin, calcitonin, or isoproterenol. The yield of basal-lateral plasma membranes was 5 to 10 percent of the amount initially present in the homogenate. The method is also applicable to the pig kidney.

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Adenylyl Cyclases Animals Cell Fractionation Cell Membrane Centrifugation, Density Gradient Kidney Cortex Kinetics Male Rats Sodium-Potassium-Exchanging ATPase Journal Article Research Support, Non-U.S. Gov't

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V Scalera, Y K Huang, B Hildmann, H Murer. 1981. A simple isolation method for basal-lateral plasma membranes from rat kidney cortex.. https://doi.org/10.3109/09687688109065422

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