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Restriction and modification in Bacillus subtilis: gene coding for a BsuR-specific modification methyltransferase in the temperate bacteriophage phi 3T.

M Noyer-Weidner·B Pawlek·S Jentsch·U Günthert·T A Trautner
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Abstract

The resistance of Phi3T DNA to degradation by the restriction enzyme BsuR or its isoschizomer HaeIII is due to obligatory modification of such DNA. Biochemical and genetical experiments indicate that Phi3T codes for a methyltransferase, which methylates Phi3T DNA itself or heterologous DNA at target sites 5'-GG(*)CC.

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Bacillus subtilisBacteriophagesDNA Restriction EnzymesDNA, ViralDNA-Cytosine MethylasesDeoxyribonucleases, Type II Site-SpecificGenes, ViralMethyltransferasesJournal ArticleResearch Support, Non-U.S. Gov't

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M Noyer-Weidner, B Pawlek, S Jentsch, U Günthert, T A Trautner. 1981. Restriction and modification in Bacillus subtilis: gene coding for a BsuR-specific modification methyltransferase in the temperate bacteriophage phi 3T.. https://doi.org/10.1128/jvi.38.3.1077-1080.1981

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