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Poly(A) polymerase activity in human rotavirus.

M Gorziglia·J Esparza
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Abstract

A poly(A) polymerase enzymic activity was found in partially purified preparations of a human rotavirus. The activity was demonstrated using conditions similar to those utilized for the detection of the poly(A) polymerase previously described in reovirus (incubation at 43 degrees C in 70 mM-tris buffer pH 7.5 containing 12 mM-Mn2+). The enzymic activity was associated only with complete, double-shelled particles. Characterization of the poly(A)-containing product of the in vitro reaction by gel filtration on Sephadex G-100, followed by chromatography on DEAE-cellulose in the presence of 7 M-urea, showed that it is composed of oligonucleotides of a chain length similar to, or slightly larger than, those observed in reovirus.

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Adenosine TriphosphateDNA-Directed RNA PolymerasesHumansNucleotidyltransferasesOligonucleotidesPoly APolynucleotide AdenylyltransferaseReoviridaeRotavirusVirionJournal Article

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M Gorziglia, J Esparza. 1981. Poly(A) polymerase activity in human rotavirus.. https://doi.org/10.1099/0022-1317-53-2-357

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