Indomethacin causes a simultaneous decrease of both prolactin binding and fluidity of mouse liver membranes.

R A Knazek; S C Liu; J R Dave; R J Christy; J A Keller

doi:10.1016/0161-4630(81)90072-0

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Indomethacin causes a simultaneous decrease of both prolactin binding and fluidity of mouse liver membranes.

R A Knazek·S C Liu·J R Dave·R J Christy·J A Keller

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Abstract

Indomethacin suppressed the numbers of prolactin receptors detectable in the liver membranes of both male and female C3H mice. This occurred in a dose-dependent fashion with 7.5 mug/gm body weight injected every 4 hours exerting a maximal effect within 20 hours. While injection of 50 mug prolactin every 4 hr increased the number of prolactin receptors in control animals it could not in the indomethacin-treated animals. Membrane fluidity was estimated by fluorescence polarization techniques using the lipid probe 1, 6-diphenylhexatriene. Indomethacin caused a decrease in membrane fluidity, whereas, exogenous prolactin increased the fluidity of the recipients' liver membranes but again could not overcome these suppressive effects of indomethacin. The data suggest that prolactin induces its own membrane-associated receptor by means of the prostaglandin cascade, perhaps by altering the fluidity of the supporting lipid bilayer.

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Animals Dose-Response Relationship, Drug Female Indomethacin Kinetics Liver Male Membrane Fluidity Mice Mice, Inbred C3H Prolactin Receptors, Cell Surface Receptors, Prolactin Journal Article

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R A Knazek, S C Liu, J R Dave, R J Christy, J A Keller. 1981. Indomethacin causes a simultaneous decrease of both prolactin binding and fluidity of mouse liver membranes.. https://doi.org/10.1016/0161-4630(81)90072-0

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