pED100, a conjugative F plasmid derivative without insertion sequences.

N Willetts; D Johnson

doi:10.1007/bf00293948

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pED100, a conjugative F plasmid derivative without insertion sequences.

N Willetts·D Johnson

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Abstract

The largest HindIII fragment of F includes the entire replication and transfer regions, and its circularisation with ligase gave the conjugative plasmid pED100. This plasmid, which contains none of the F insertion sequences, was essentially unable to mobilise the E. coli chromosome or to give integrative suppression of a dnaA strain.

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Chromosome Mapping Conjugation, Genetic DNA Restriction Enzymes DNA Transposable Elements DNA, Bacterial Escherichia coli F Factor Recombination, Genetic Journal Article

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N Willetts, D Johnson. 1981. pED100, a conjugative F plasmid derivative without insertion sequences.. https://doi.org/10.1007/bf00293948

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