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PubMed · 6274738

[Lambda H-lambda T 80 hybrid study of the DNA structural gene region in lambda and phi 80 phages].

A S Boĭtsov·O N Shalaeva·I G Kochnev·V N Rybchin
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Abstract

Hybrids lambda H lambda T80 are formed due to recombination of the phage lambda att80 and phi 80 prophage partially deleted in the region of structural genes. Genetic structure of 22 independently isolated lambda H lambda T80 hybrids was determined by the restriction method and it was shown that recombination took place in the genes A, C, D and H. The frequencies of hybrid formation diminish from 1.10(-3) to 4.10(-5) for this gene order, which suggests that the polar divergence of nucleotide sequencies in the region of structural genes exists. It was found that formation of hybrids with recombination in the region of "weak" homology (gene H) was possible only when the region of "strong" homology was present in the deleted phi 80 prophage to initiate recombination.

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Bacteriophage lambdaColiphagesCrosses, GeneticCrossing Over, GeneticDNA Restriction EnzymesDNA, ViralGenesGenes, ViralRecombination, GeneticEnglish AbstractJournal Article

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A S Boĭtsov, O N Shalaeva, I G Kochnev, V N Rybchin. 1981. [Lambda H-lambda T 80 hybrid study of the DNA structural gene region in lambda and phi 80 phages].. https://pubmed.ncbi.nlm.nih.gov/6274738/

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