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PubMed · 6277976

Techniques for virus detection in aquatic sediments.

G Bitton·Y J Chou·S R Farrah
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Abstract

Laboratory experiments have been undertaken to study the adsorption-desorption of poliovirus to and from marine and freshwater sediments. It was observed that marine sediments retained 99% of added virus, whereas, freshwater sediments adsorbed approximately 40% of the added virus. Ten eluents were investigated for their ability to desorb viruses from a marine sediment. It was found that virus elution from the marine sediment was relatively low and ranged from less than 1% to 44%. Two eluents, urea-lysine and TCA-glycine at pH 9.0, were found to be the most efficient among the eluents tested. Viruses were more easily released from freshwater than from marine sediments. When urea-lysine, beef extract and purified casein were used as eluents, the overall virus recovery ranged from 8% to 22% for the marine sediment and from 23% to 59% for the freshwater sediments. The urea-lysine methods was used for the detection of indigenous enteroviruses in sediments from a closed shellfish bed. Enteroviruses were recovered with concentrations up to 708 TCID50/50 g of sediment.

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AdsorptionEnterovirusFresh WaterLysineMethodsPoliovirusSeawaterSoil MicrobiologyUreaWater MicrobiologyJournal ArticleResearch Support, U.S. Gov't, Non-P.H.S.

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G Bitton, Y J Chou, S R Farrah. 1982. Techniques for virus detection in aquatic sediments.. https://doi.org/10.1016/0166-0934(82)90048-9

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