Complement-fixation test for rotavirus detection: comparison and analysis of different methods to reduce anti-complementary activity of some specimens.

P Pauri; P Bagnarelli; M Clementi

doi:10.1016/0166-0934(82)90037-4

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Complement-fixation test for rotavirus detection: comparison and analysis of different methods to reduce anti-complementary activity of some specimens.

P Pauri·P Bagnarelli·M Clementi

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Abstract

The complement-fixation test may be used to detect rotaviral antigens directly in clinical specimens. However, a certain number of specimens tested for human rotaviruses by the complement-fixation test show an anti-complementary activity. By comparing eight techniques we analysed this anti-complementary activity and identified the best method for its reduction. Pretreatment of clarified supernatant of stool suspensions by some methods resulted in a reduction of anti-complementary activity, without reducing the sensitivity of the method. Clarified supernatants of 8/36 (22.2%) specimens were anti-complementary; this anti-complementary activity was best removed by absorption with fetal calf serum or calf albumin. Such treatment offers practical means of increasing the specificity of complement-fixation test. Some observations suggest that the anti-complementary activity of stool suspensions may be frequently due to presence of one or more chelating agents that may be in faecal specimens.

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Absorption Animals Antigens, Viral Cattle Centrifugation Complement Fixation Tests Complement Inactivator Proteins Enzyme-Linked Immunosorbent Assay Feces Guinea Pigs Humans Reoviridae Rotavirus Serum Albumin Specimen Handling Comparative Study Journal Article

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P Pauri, P Bagnarelli, M Clementi. 1982. Complement-fixation test for rotavirus detection: comparison and analysis of different methods to reduce anti-complementary activity of some specimens.. https://doi.org/10.1016/0166-0934(82)90037-4

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