Is Percoll innocuous to cells?

J S Wakefield; J S Gale; M V Berridge; T W Jordan; H C Ford

doi:10.1042/bj2020795

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Is Percoll innocuous to cells?

J S Wakefield·J S Gale·M V Berridge·T W Jordan·H C Ford

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Abstract

Peritoneal macrophages from mice, isolated rat liver Kupffer cells and rat testis Leydig cells ingested large numbers of Percoll particles, a gradient medium widely used for separation of cells and subcellular organelles by density-gradient centrifugation. A decrease in the percentage of macrophages adhering to plastic also occurred after exposure of the cells to Percoll, even at 4 degrees C, a temperature at which Percoll was not ingested. The effect of Percoll on macrophage adherence may involve a loose association between the density medium and the cell surface. Other cell-surface-related phenomena may also be affected by prior exposure of cells to Percoll.

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Animals Ascitic Fluid Cell Adhesion Colloids Female In Vitro Techniques Kupffer Cells Leydig Cells Liver Macrophages Male Mice Mice, Inbred CBA Microscopy, Electron Povidone Rats Silicon Dioxide Journal Article

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J S Wakefield, J S Gale, M V Berridge, T W Jordan, H C Ford. 1982-03-15. Is Percoll innocuous to cells?. https://doi.org/10.1042/bj2020795

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