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Asynchronous bidirectional replication of polyoma virus DNA.

A J Buckler-White·M R Krauss·V Pigiet·R M Benbow
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Abstract

The structure of polyoma virus replicative intermediates isolated from infected 3T6 cells was analyzed by two-dimensional agarose gel electrophoresis (Sundin and Varshavsky, Cell 21:103-114, 1980) and quantitative electron microscopy (Krauss and Benbow, J. Virol. 38:815-825, 1981). DNA replication was initiated at a single site (ori) in essentially all of the replicative intermediates. Most of the early replicative intermediates were formed by unidirectional synthesis in the direction of early transcription. Most mid- and late replicative intermediates contained two replication forks which had traveled unequal distances from the origin. Asynchronous initiation of the two growing forks was postulated to account for these observations.

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AnimalsCells, CulturedDNA ReplicationDNA, ViralElectrophoresis, Agar GelMiceMicroscopy, ElectronPolyomavirusSimian virus 40Virus ReplicationJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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A J Buckler-White, M R Krauss, V Pigiet, R M Benbow. 1982. Asynchronous bidirectional replication of polyoma virus DNA.. https://doi.org/10.1128/jvi.43.3.885-895.1982

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