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A new restriction endonuclease from Citrobacter freundii.

A A Janulaitis·P S Stakenas·E N Lebedenko·Berlin YuA
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Abstract

CfrI, a new restriction endonuclease of unique substrate specificity, has been isolated from a Citrobacterfreundii strain. The enzyme recognizes a degenerated sequence PyGGCCPu in double-strand DNA and cleaves it between Py and G residues to yield 5' -protruding tetranucleotide ends GGCC.

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Base SequenceCitrobacterDNA Restriction EnzymesKineticsSubstrate SpecificityJournal Article

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A A Janulaitis, P S Stakenas, E N Lebedenko, Berlin YuA. 1982-10-25. A new restriction endonuclease from Citrobacter freundii.. https://doi.org/10.1093/nar%2F10.20.6521

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