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Poly(ADP-ribose) polymerase auto-modification and interaction with DNA: electron microscopic visualization.

G de Murcia·J Jongstra-Bilen·M E Ittel·P Mandel·E Delain
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Abstract

The interaction between purified calf thymus poly(ADP-ribose) polymerase and its activating co-purified DNA (sDNA) was investigated by electron microscopy. We have shown that the enzyme-DNA complex possesses a nucleosome-like structure. The enzyme-bound DNA (sDNA) was found to be enriched in single-stranded regions and branched structures, presumed to be replication forks. The auto-ribosylated polymerase as well as the branched poly(ADP-ribose) formed were visualized by dark field electron microscopy during the auto-ADP-ribosylation reaction and the possible mechanism of this phenomenon is discussed.

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AnimalsCattleDNADNA ReplicationDNA, Single-StrandedMicroscopy, ElectronNAD+ NucleosidasePoly(ADP-ribose) PolymerasesProtein Processing, Post-TranslationalJournal ArticleResearch Support, Non-U.S. Gov't

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G de Murcia, J Jongstra-Bilen, M E Ittel, P Mandel, E Delain. 1983. Poly(ADP-ribose) polymerase auto-modification and interaction with DNA: electron microscopic visualization.. https://doi.org/10.1002/j.1460-2075.1983.tb01460.x

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