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Nucleoside 5'-(beta, gamma-peroxytriphosphates).

K J Gibson·N J Leonard
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Abstract

Procedures for the synthesis, purification, and characterization of beta, gamma-peroxy analogues of the eight common ribo- and deoxyribonucleoside triphosphates have been developed. Although adenosine 5'-(beta, gamma-peroxytriphosphate) was stable to conditions in most biochemical systems, incubation of a solution of the analogue at 100 degrees C led to formation of AMP and ATP, as well as ADP. NAD+ pyrophosphorylase was the only enzyme among 13 tested for which adenosine 5'-(beta, gamma-peroxytriphosphate) was a good substrate, but the analogue was an effective inhibitor for a number of kinases. The peroxy compounds tested inactive with Escherichia coli RNA polymerase and DNA polymerase I, as well as with wheat germ RNA polymerase II.

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Adenosine TriphosphateAnimalsDNA Polymerase IDNA-Directed RNA PolymerasesEscherichia coliIndicators and ReagentsLiverLuciferasesMusclesPhosphotransferasesPlantsSalmonella typhimuriumStructure-Activity RelationshipJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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K J Gibson, N J Leonard. 1984-01-03. Nucleoside 5'-(beta, gamma-peroxytriphosphates).. https://doi.org/10.1021/bi00296a013

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