3T3 variants unable to bind epidermal growth factor cannot complement in co-culture.

E Terwilliger; H R Herschman

doi:10.1016/0006-291x(84)91067-2

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3T3 variants unable to bind epidermal growth factor cannot complement in co-culture.

E Terwilliger·H R Herschman

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Abstract

A Swiss-Webster 3T3 variant, called 3T3-ENR7, unable to divide in response to epidermal growth factor was isolated by the mitogen-colchicine selection technique. Like the other EGF non-proliferative variants (3T3-NR6 and 3T3-TNR2), 3T3-ENR7 was unable to bind 125I-EGF. Pairwise co-culture of the three independently isolated EGF non-responsive variants did not restore mitogenic responsiveness to EGF.

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Animals Cell Division Cells, Cultured Epidermal Growth Factor ErbB Receptors Genetic Variation Kinetics Mice Receptors, Cell Surface Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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E Terwilliger, H R Herschman. 1984-01-13. 3T3 variants unable to bind epidermal growth factor cannot complement in co-culture.. https://doi.org/10.1016/0006-291x(84)91067-2

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