Anti-D prozone and membrane sulfhydryl modification.

J T Jameson; J E Kleeman; S P Masouredis; E J Victoria

doi:10.1046/j.1537-2995.1984.24284173343.x

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Anti-D prozone and membrane sulfhydryl modification.

J T Jameson·J E Kleeman·S P Masouredis·E J Victoria

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Abstract

An IgG anti-D prozone is produced by progressive inactivation of the D antigen following red cell exposure to increasing concentrations of thimerosal and phenol present as antibody excess is achieved. Partial inactivation of the D antigen by routinely added thimerosal, an organic mercurial, and phenol is associated with an unstable D antigen-antibody complex resulting in an increased rate of dissociation of anti-D and with a decreased reactivity of the cell-bound anti-D in the antiglobulin reaction. Complete D inactivation occurs with concentrations in excess of 0.43 microM thimerosal. If attributable to inactivation of a surface-exposed sulfhydryl group, it suggests that less than 5 percent of these are involved in D-antigen activity. The data do not exclude the possibility that D inactivation may result from alteration of sulfhydryl groups other than those exposed at the surface.

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Antibody Affinity Binding Sites, Antibody Coombs Test Erythrocyte Membrane Hemagglutination Humans Immunoglobulins Kinetics Phenol Phenols Rh-Hr Blood-Group System Rho(D) Immune Globulin Sulfhydryl Compounds Thimerosal Journal Article Research Support, U.S. Gov't, P.H.S.

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J T Jameson, J E Kleeman, S P Masouredis, E J Victoria. Anti-D prozone and membrane sulfhydryl modification.. https://doi.org/10.1046/j.1537-2995.1984.24284173343.x

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