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PubMed · 6337995

dnaB analog function associated with plasmid R100drd-1.

A A Potter·V N Iyer
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Abstract

Plasmid R100 and a number of its derivatives were able to suppress the temperature sensitivity of strains carrying different alleles of the dnaB gene of Escherichia coli K-12. R100drd-l and pAR132 were able to rescue a strain carrying the dnaB266(Am) mutation in the absence of any known amber suppressors. This was taken as evidence for the existence of an R100drd-l dnaB analog function. The R100drd-l dnaB analog was different from those of bacteriophages P1 and P7 in that it was able to support the growth of bacteriophage lambda in a dnaB266(Am) background. The dnaB analog was also shown to be thermosensitive. The structural gene for this protein lies within the EcoRI fragment D of R100drd-l.

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DNA, BacterialEscherichia coliGenes, BacterialPlasmidsJournal ArticleResearch Support, Non-U.S. Gov't

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A A Potter, V N Iyer. 1983. dnaB analog function associated with plasmid R100drd-1.. https://doi.org/10.1128/jb.153.3.1308-1314.1983

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