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Gene for staphylococcal protein A.

S Löfdahl·B Guss·M Uhlén·L Philipson·M Lindberg
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Abstract

The gene for protein A from Staphylococcus aureus was cloned into pBR322 in Escherichia coli. An immunoassay was used to detect production of the protein. Protein A produced in E. coli was found in the periplasmic space and was purified and concentrated by IgG-Sepharose affinity chromatography. DNA sequence assay of the gene revealed a region with the general features of a prokaryotic signal peptide and a fifth structural region homologous to the four repetitive regions found earlier by amino acid sequence determination of the mature protein. Upstream from the structural gene there is a possible promoter region and a ribosomal binding sequence typical of gram-positive bacteria. The initiation codon is TTG.

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Base SequenceChromosome MappingCloning, MolecularEscherichia coliGenesGenes, BacterialStaphylococcal Protein AStaphylococcus aureusJournal ArticleResearch Support, Non-U.S. Gov't

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S Löfdahl, B Guss, M Uhlén, L Philipson, M Lindberg. 1983. Gene for staphylococcal protein A.. https://doi.org/10.1073/pnas.80.3.697

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