[Nuclear DNAse from rat brain].

V A Ivanov; O N Terpilovskaia; T M Tret'iak

PubMed · 6342244

[Nuclear DNAse from rat brain].

V A Ivanov·O N Terpilovskaia·T M Tret'iak

Abstract

Nuclear DNase specific for single-stranded DNA was obtained from the rat brain. The enzyme was isolated from a soluble protein fraction of total cell nuclei using gel filtration and ion-exchange chromatography. Nuclear DNase is Mg2+-dependent exodeoxyribonuclease and hydrolyzes homological, heterological and synthetic substrate at the same rate. It is established that nucleoside-5'-monophosphates are the major product of native and synthetic polydeoxyribonucleotides hydrolysis. It is supposed that the enzyme participates in reparation of neuron DNA.

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Animals Brain Cattle Cell Fractionation Cell Nucleus Chromatography, Gel DNA DNA, Bacterial DNA, Single-Stranded Deoxyribonucleases Escherichia coli Hydrolysis Male RNA, Fungal Rats Rats, Inbred Strains Substrate Specificity Thymus Gland Journal Article

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V A Ivanov, O N Terpilovskaia, T M Tret'iak. [Nuclear DNAse from rat brain].. https://pubmed.ncbi.nlm.nih.gov/6342244/

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