Enzyme immunoassay for methamphetamine.

K Aoki; Y Kuroiwa

doi:10.1248/bpb1978.6.33

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Enzyme immunoassay for methamphetamine.

K Aoki·Y Kuroiwa

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Abstract

A competitive enzyme immunoassay for methamphetamine with alkaline phosphatase labeled methamphetamine, Sepharose-antibody and p-nitrophenylphosphate as substrate was developed. The anti-methamphetamine antisera produced in rabbits by immunization with N-(4-aminobutyl) methamphetamine-BSA conjugate were specific for methamphetamine and showed low cross-reactivities with p-OH methamphetamine and amphetamine (metabolites of methamphetamine). The range of methamphetamine measurable by the enzyme immunoassay was 1 to 300 ng/tube. According to the assay, methamphetamine could be detected from urine and extract of hair.

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Alkaline Phosphatase Antibody Formation Fluorescent Antibody Technique Hair Humans Immunoenzyme Techniques Immunoglobulin G Methamphetamine Substance-Related Disorders Journal Article

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K Aoki, Y Kuroiwa. 1983. Enzyme immunoassay for methamphetamine.. https://doi.org/10.1248/bpb1978.6.33

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