Splice junctions: association with variation in protein structure.

C S Craik; W J Rutter; R Fletterick

doi:10.1126/science.6344214

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PubMed · 6344214

Splice junctions: association with variation in protein structure.

C S Craik·W J Rutter·R Fletterick

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Abstract

A comparison between eukaryotic gene sequences and protein sequences of homologous enzymes from bacterial and mammalian organisms shows that intron-exon junctions frequently coincide with variable surface loops of the protein structures. The altered surface structures can account for functional differences among the members of a family. Sliding of the intron-exon junctions may constitute one mechanism for generating length polymorphisms and divergent sequences found in protein families. Since intron-exon junctions map to protein surfaces, the alterations mediated by sliding of these junctions can be effected without disrupting the stability of the protein core.

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Amino Acid Sequence Animals Bacterial Proteins Base Sequence Biological Evolution DNA Endopeptidases Eukaryotic Cells Genes Genes, Bacterial Protein Conformation Proteins Serine Endopeptidases Tetrahydrofolate Dehydrogenase Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

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C S Craik, W J Rutter, R Fletterick. 1983-06-10. Splice junctions: association with variation in protein structure.. https://doi.org/10.1126/science.6344214

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