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PubMed · 6354642

Somatic cell genetics and flow cytometry.

M E Kamarck·J A Barbosa·L Kühn·P G Peters·L Shulman·F H Ruddle
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Abstract

Human genes coding cell surface molecules can be introduced into mouse host cells using a variety of somatic cell genetic techniques. Because these human gene products can be detected using indirect immunofluorescence on viable cells, the genes themselves can be monitored and manipulated using flow cytometry and sorting. In this paper, we review ways that we have used cell sorting to develop a somatic cell genetic analysis of the human cell surface.

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AnimalsAntigens, SurfaceCell FusionCell SeparationCloning, MolecularFlow CytometryFluorescent Antibody TechniqueGene Expression RegulationGenesGenetic TechniquesHLA AntigensHumansHybrid CellsInterferon Type IMiceTransfectionbeta 2-MicroglobulinJournal ArticleResearch Support, U.S. Gov't, P.H.S.Review

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M E Kamarck, J A Barbosa, L Kühn, P G Peters, L Shulman, F H Ruddle. 1983. Somatic cell genetics and flow cytometry.. https://doi.org/10.1002/cyto.990040202

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