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A simple method for immunoselective cell separation with the avidin-biotin system.

J Wormmeester·F Stiekema·K De Groot
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Abstract

A new method for immunoselective cell separation is described which uses biotinylated antibodies and avidin-coupled sheep erythrocytes for rosette formation, and a density gradient to separate the rosetting from the non-rosetting cells. The utility of the technique is illustrated by separating subpopulations of rabbit, mouse and rat lymphocytes. The method offers a rapid, sensitive and easily reproducible means for obtaining purified cell populations.

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AnimalsAvidinBinding Sites, AntibodyBiotinCell SeparationErythrocytesFemaleFluorescent Antibody TechniqueLymphocytesMaleMiceRabbitsRatsRats, Inbred StrainsRosette FormationJournal Article

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J Wormmeester, F Stiekema, K De Groot. 1984-03-16. A simple method for immunoselective cell separation with the avidin-biotin system.. https://doi.org/10.1016/0022-1759(84)90478-2

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