PubMed · 6371769
The source did not provide an abstract. Follow the original record for more information.
Explore connections, maps & timelines
S Someya. 1983. [Agar-alginate continuous impression taking].. https://pubmed.ncbi.nlm.nih.gov/6371769/
Cite the original work for its findings. Save a collection to share your selection of sources.
KEEP EXPLORING
Highly methylated agars were isolated from the red seaweed, Gracilaria eucheumoides, harvested in Japan. One of the obtained agars formed a thermo-reversible gel with a high melting point up to 121 degrees and was indicated to consist of a regularly repeating structure,-->3 6-O-methyl-beta-D-Gal(1-->4) 3,6-anhydro-2-O-methyl-alpha-L-Gal 1-->.
Thirty strains of T. hamatum, T. harzianum, T. koningii, T. longibrachiatum and T. viride were selected for in vitro mycoparasitic activity against Sclerotinia minor. High destruction of sclerotia (up to 70% in two weeks) was caused by antagonists only at 25 degrees C, in autoclaved soil, because the optimum temperature for radial growth of Trichoderma strains (25-30 degrees C) was significantly higher than those of phytophatogenic fungus (20 degrees C). The most active strains, which were selected also for competitive saprophytic ability could enhance the percentage of decayed sclerotia 5-9 fold over the control even after 1 week under non sterile conditions. A silicon rubber fixation technique was developed for recovery of irregularly shaped S. minor sclerotia.
Autografts using untreated or in vitro manipulated bone marrow and peripheral blood stem cells represent promising approaches to the treatment of malignant diseases. In this work, the collagen gel culture technique was compared with agar and methylcellulose for its capacity to permit the growth of human granulomonocytic (day 14 CFU-GM; collagen vs agar or MTC) or erythroblastic (day 7 CFU-E and day 14 BFU-E; collagen versus methylcellulose) colonies in autologous transplantation products. Our results show that the collagen culture system always gave as many or more colonies than the other techniques. It also allowed harvesting of gels onto glass slides and subsequent May-Grünwald-Giemsa, cytochemical or immunocytochemical staining. We suggest that the collagen assay represents an interesting alternative to the widely used agar or methylcellulose systems for the culture of hematopoietic progenitors because of the equal or higher number of colonies detected, the easy phenotypical identification of colonies in stained gels, and the ability to store high-quality documentation. This technique is particularly attractive for use in the quality control of autologous bone marrow transplantation procedures.