A new microtechnique for quantitating cell movement in vitro using polystyrene bead monolayers.

J L Obeso; R Auerbach

doi:10.1016/0022-1759(84)90180-7

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A new microtechnique for quantitating cell movement in vitro using polystyrene bead monolayers.

J L Obeso·R Auerbach

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Abstract

In this paper we describe a new and simple technique for studying cell movement in vitro. This method is based upon the migration of endothelial cells on polystyrene bead monolayers. It permits the rapid preparation and screening of multiple 96-well plates for effects on migration, requires fewer samples per test and has the potential for precise quantitation by digital image analysis. The locomotive response of endothelial cells in response to different protein matrices (fibrin, collagen, albumin, gelatin), as used by this technique, is also described. Experiments in progress have shown that this technique can be used to study the effect of different factors (monoclonal antibodies, lymphokines, etc.) on cell migration, and that it may be used with other cell types which exhibit cell migration on substrates in vitro.

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Animals Blood Proteins Cattle Cell Movement Cells, Cultured Culture Media Endothelium Fetal Blood Immunologic Techniques Mice Microspheres Phagocytosis Polystyrenes Journal Article Research Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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J L Obeso, R Auerbach. 1984-05-25. A new microtechnique for quantitating cell movement in vitro using polystyrene bead monolayers.. https://doi.org/10.1016/0022-1759(84)90180-7

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