PubMed · 6392959
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P N Baer. 1984. The Keyes technique. A commentary.. https://pubmed.ncbi.nlm.nih.gov/6392959/
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OBJECTIVES: To detect any differences in the propensity of individual saliva to cause in vitro staining by chlorhexidine and tea. METHODS: Unstimulated human saliva was collected on a daily basis and used to coat optically clear Perspex specimens. Specimens were subjected to the original chlorhexidine/tea staining model described by Prayitno and Addy (Journal of Periodontal Research 1979;14:397-402), and cycles repeated until a maximum optical density of two was reached. RESULTS: Stain development increased incrementally with increasing cycles. Differences in chlorhexidine/tea staining between subjects were obvious by cycle 3 where the lowest 'stainer' had 56% less stain than the highest 'stainer'. Highly significant differences between subjects were seen during staining cycles 3-6, but not at cycles 7 and 8. CONCLUSIONS: In vitro stain formation using saliva from different individuals occurred at differing rates when all other variables were standardised. The properties of saliva accounting for this are still unknown, and warrant further study.
OBJECTIVE: Secretory otitis media is defined by a chronic effusion in the middle ear cavities, behind an intact tympanic membrane without acute infection. In the course of secretory otitis media, the effusion is sometimes thick (or mucoid) and other times thin (or serous). For several authors, these differences might be related to different inflammatory levels rather than to distinct pathophysiological mechanisms. The purpose of this study was to determine whether the modulation of ion transport by inflammation could account for the differences observed in the aspect of ME effusion. METHODS: Hydrogen peroxide (H2O2), used as an inflammation model was tested on a middle ear cell line (MESV) in culture. RESULTS: Results show that low and high concentrations of H2O2 have opposite effects on ion (and then water) absorption by the middle ear epithelium. CONCLUSION: the modulation of ion and water absorption by inflammation could modify the mucins concentration in the effusion, and explain observed viscosity differences in the course of secretory otitis media.
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